Long Island K-9 Service was created by Officer James Greco who began training canines in 1973. Featuring his trained search and detection dogs, Officer Greco initially presented a "Zero Tolerance towards Drugs" program to school-age children throughout Long Island, and the five boroughs. The program ultimately brought him local media attention from television's Inside Edition and News Channel 12. Private investigation agencies, major hotel chains, and entertainment "hot spots" also began to contact him for his services. The company quickly grew to include explosive detection, private and commercial security, and protection services.

In 1999, Officer Greco was called to testify as a canine expert in the case of Orlando Diaz vs. Bloomfield, New Jersey. His testimony not only helped win the case for the defendant, but it also saved a dog named Duke from being destroyed. This dog-bite case was featured on television's New York News Channel 4.

In 2001, a Long Island firm specializing in environmental cleanups contracted Officer Greco to train three Labrador Retrievers for the detection of hazardous mold spores. Upon completion of this training, he was invited to present a live demonstration with these dogs, including a Q&A, to an audience of 1,000 insurance executives in Charlotte, NC. This same company was utilized on 911, at the World Trade Center.

Long Island K-9 Service is licensed with the Drug Enforcement Agency (DEA) as a certified training facility and has trained with the United States Police Canine Association, the California Narcotics Canine Association, and has trained with the New York State, Kingston Police Department